Single Nucleotide Polymorphism Mapping and Alignment of Recombinant Chromosome Substitution Lines in Barley
نویسندگان
چکیده
منابع مشابه
Single-nucleotide polymorphism mapping.
Single-nucleotide polymorphism (SNP) mapping is the easiest and most reliable way to map genes in Caenorhabditis elegans. SNPs are extremely dense and usually have no associated phenotype, making them ideal markers for mapping. SNP mapping has three steps. First, recombinant mutant animals are generated over a polymorphic strain (usually CB4856) using standard genetic techniques. Second, the ge...
متن کاملMapping barley genes to chromosome arms by transcript profiling of wheat-barley ditelosomic chromosome addition lines.
Wheat-barley disomic and ditelosomic chromosome addition lines have been used as genetic tools for a range of applications since their development in the 1980s. In the present study, we used the Affymetrix Barley1 GeneChip for comparative transcript analysis of the barley cultivar Betzes, the wheat cultivar Chinese Spring, and Chinese Spring - Betzes ditelosomic chromosome addition lines to phy...
متن کاملTranscriptome analysis and physical mapping of barley genes in wheat-barley chromosome addition lines.
Wheat-barley chromosome addition lines are useful genetic resources for a variety of studies. In this study, transcript accumulation patterns in Betzes barley, Chinese Spring wheat, and Chinese Spring-Betzes chromosome addition lines were examined with the Barley1 Affymetrix GeneChip probe array. Of the 4014 transcripts detected in Betzes but not in Chinese Spring, 365, 271, 265, 323, 194, and ...
متن کاملSingle Nucleotide Polymorphism Analysis of Protamine Genes in Infertile Men
Background Single nucleotide polymorphism (SNPs) are considered as one of the underlying causes of male infertility. Proper sperm chromatin packaging which involves replacement of histones with protamines has profound effect on male fertility. Over 20 SNPs have been reported for the protamine 1 and 2. MaterialsAndMethods The aim of this study was to evaluate the frequency of two previously repo...
متن کاملLinkage mapping of a single nucleotide polymorphism (SNP) in the porcine QDPR gene to chromosome 8.
Source ⁄ description of primers: Quinoid dihydropteridine reductase (QDPR) catalyzes the NADH-mediated reduction of quinonoid dihydrobiopterin and is an essential component of the pterin-dependent aromatic amino acid hydroxylating systems. A cDNA clone containing the full coding region of the porcine QDPR (GenBank accession no. AF526879) has been isolated from the Meat Animal Research Center (M...
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ژورنال
عنوان ژورنال: Plant and Cell Physiology
سال: 2011
ISSN: 1471-9053,0032-0781
DOI: 10.1093/pcp/pcr024